Fig. 4
Activated microglia in white matter. a 10× photomicrographs of representative OX-6 immunolabelled activated microglial cells in the corpus callosum, internal capsule and fimbria hippocampi from the boxed regions indicated on the whole brain section insertion, right hemisphere. Photographs of the activated microglia in the three white matter regions of the three-month-old TG rat are shown in the right column. Scale bar 200μm. b Integrated density as a measure of microgliosis for corpus callosum and internal capsule. Animal numbers are as follows: control WT (n = 12), control TG (n = 11), HCD WT (n = 12), HCD TG (n = 11). Area coverage by a positive signal (as a percentage of a total area of the region) as a measure of microgliosis for fimbria. Animal numbers are n = 4 in each group. c OX-6 positive activated microglia cell counts in a field of area 100 mm2 in the in the corpus callosum, internal capsule and fimbria hippocampi. Values are presented as mean ± SEM. Significance is indicated by * between HCD TG and all other groups; by † between control groups; by ‡ between control TG and HCD WT groups. One-way ANOVA and Tukey’s multiple comparisons test, p < 0.05. 3M three-month-old TG rat, HCD hypercaloric diet, TG transgenic, WT wildtype