Fig. 3

APP/PS1 pathology and high-fat diet cooperatively promote cytokine expression. a Luminex analysis of 22 cytokines (columns, z-scored) expressed in the cortex of APP/PS1, HFD, and APP/PS1-HFD mice (each row is a cortex sample). b PLSDA identified two profiles of cytokines, LV1 and LV2, that distinguished groups. LV1 separates APP/PS1-HFD mice (positive) from both APP/PS1 and HFD mice (negative). LV2 separates HFD mice (positive) from APP/PS1 and APP/PS1-HFD mice (negative). c The weighted profile of cytokines representing LV1. Errors bars on each cytokine were computed by PLSDA model regeneration using iterative subsampling of 80% of samples (mean ± SD). d Scoring the data for each sample in a on LV1 revealed that combined APP/PS1-HFD pathology cooperatively increased the LV1 cytokine profile compared to either APP/PS1 or db/db pathology alone (**p < 0.01, Welch’s ANOVA with Dunnett’s T3 test). e The weighted profile of cytokines representing LV2. Errors bars on each cytokine were computed by PLSDA model regeneration using iterative subsampling of 80% of samples (mean ± SD). f Scoring the data for each sample in b on LV2 revealed that HFD is significantly upregulated on the LV2 cytokine profile compared to both APP/PS1 and APP/PS1-HFD (**p < 0.01, Welch’s ANOVA with Dunnett’s T3 test). Data were collected from 21 animals (11 M/10F, HFD 4 M/3F, APP/PS1 3 M/4F, APP/PS1-HFD 4 M/3F)