Fig. 5

Double immunofluorescence staining of NGF expression on different cell types of the nervous and immune system. a, b DIF of NGF and NeuN in the grey matter of spinal cord at acute and chronic phase of the EAE model. c Graphical representation of the percentage of co-expression during the EAE course. d, e DIF of NGF and GAP-43, g, h DIF of NGF and GFAP, j, k DIF of NGF and Arginase 1, m, n DIF of NGF and Mac-3, and p, q DIF of NGF and B220, in the inflammatory loci of the white matter of the spinal cord during the acute and chronic phase of the EAE model. f, i, l, o, r Their graphical representations of the percentage of co-localization at the acute and chronic phase of the EAE model. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar = 100 μm