Fig. 1

The expression of miR-7 is upregulated in BTI model. a Schematic representation of the animal experiments. b WT mice (n = 6 per group) were intraperitoneally injected with LPS (2.5 mg/kg of body weight) and control group with PBS. After 12 h, the pathology of brain tissue was observed by H&E staining (n = 3 per group; arrows indicate hemorrhage foci). c–f The relative expression levels of cytokines (IL-1β, IL-6, TNF-α, and TGF-β) were detected by real-time PCR assay and calculated (n = 6 per group; t test). g The expression levels of pre-miR-7-1, pre-miR-7-2, and pre-miR-7-b in brain tissue were determined by real-time PCR assay and calculated (n = 6 per group; t test). h The expression level of pre-miR-7-2 in brain tissue was determined by FISH (n = 3 per group). i The relative expression of mature miR-7 in brain tissue was analyzed by real-time PCR assay and calculated (n = 6 per group, *P < 0.05, **P < 0.01)