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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Hypomethylation of nerve growth factor (NGF) promotes binding of C/EBPα and contributes to inflammatory hyperalgesia in rats

Fig. 3

Hypomethylation of CpG islands of the NGF gene promoter after CFA treatment. a Online software prediction of CpG islands around the NGF gene promoter region. The light blue areas on the map indicate the potential CpG islands. b The representative MSP assay showed DNA methylation and unmethylation ratio of the CpG islands in the NGF gene promoter region of L4–6 DRGs was significantly decreased after 3 days of CFA treatment (*P < 0.05, compared with the control group, n = 4). c Schematic of CpG island showing locations of the 12 CpG sites in the NGF gene promoter area d BSP sequencing, CFA injection resulted in a significant percentage of methylated CpG sites decrease to 16.7% in CpG islands of the NGF gene promoter region. e, f The expression of DNMT3b mRNA and protein in DRGs were significantly decreased after CFA treatment (3 days after CFA injection) (*P < 0.05, compared with the control group, n = 4), while DNMT3a level did not change significantly

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Journal of Neuroinflammation

ISSN: 1742-2094