Table 2 Primers used in Methylation-specific PCR and bisulfite sequencing

 NGF-MSP-M-F1:

5′-GAGTCGAGTTTTATAAGGGTGTAGC-3′

 NGF-MSP-M-R1:

5′-ACTTCTTTCAAAAATACGTTTCGAC-3′

 NGF-MSP-U-F1:

5′-TTGAGTTGAGTTTTATAAGGGTGTAGT-3′

 NGF-MSP-U-R1:

5′-ACTTCTTTCAAAAATACATTTCAAC-3′

 NGF-BSP-F:

5′-GAGTTTGGAGGAGGGGTTAAATATA 3′

 NGF-BSP-R:

5′-CCAAACAAAAAACCAAACACAC-3′