Fig. 4

Effect of preventive ADAMTS13 treatment on inflammatory factors in EAE mice. a Quantitative RT-PCR analysis of IL-1β, CXCL1, and CCL2 mRNA levels in the spinal cords of ADAMTS13- and vehicle-treated EAE mice at 22 dpi. Data represent fold changes compared to the spinal cords of naïve mice. b Supernatants obtained from spinal cord homogenates from naïve, ADAMTS13- and vehicle-treated EAE mice at 22 dpi were subjected to ELISA kits for IL-1β, IL-17, and IFN-γ. The results are expressed as inflammatory factor concentrations, averaged by the total protein contents of the supernatants. Values are expressed as the mean ± SEM (n = 5–6) and Student’s t test was used to compare differences between groups. *P < 0.05; **P < 0.01; ns, no significance