Fig. 6

Effect of preventive ADAMTS13 treatment on peripheral inflammation in EAE mice. Cells from the blood (a) and spleen (b) of ADAMTS13- or vehicle-treated EAE mice at 22 dpi were isolated and measured by flow cytometry. Leukocyte populations were identified as T lymphocytes (CD45^hi CD11b⁻ CD3⁺), neutrophils (CD45^hi CD11b⁺ Ly6C^int Ly6G^hi), or monocytes (CD45^hi CD11b⁺ Ly6C^hi Ly6G⁻). c Graph illustrating quantitative data for the percentage of inflammatory cells in total CD45⁺ cells in the blood and spleen of mice treated with vehicle or ADAMTS13 at 22 dpi. For intracellular staining, splenocytes were stimulated ex vivo for 5 h and then gated on the CD3⁺CD4⁺ population. d The percentages of CD4⁺IFN-γ⁺ and CD4⁺IL-17⁺ cells were assessed. Values are expressed as the mean ± SEM (n = 6 each group), and Student’s t test was used to compare differences between groups. *P < 0.05; ns no significance