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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: ADAMTS13 ameliorates inflammatory responses in experimental autoimmune encephalomyelitis

Fig. 6

Effect of preventive ADAMTS13 treatment on peripheral inflammation in EAE mice. Cells from the blood (a) and spleen (b) of ADAMTS13- or vehicle-treated EAE mice at 22 dpi were isolated and measured by flow cytometry. Leukocyte populations were identified as T lymphocytes (CD45hi CD11b CD3+), neutrophils (CD45hi CD11b+ Ly6Cint Ly6Ghi), or monocytes (CD45hi CD11b+ Ly6Chi Ly6G). c Graph illustrating quantitative data for the percentage of inflammatory cells in total CD45+ cells in the blood and spleen of mice treated with vehicle or ADAMTS13 at 22 dpi. For intracellular staining, splenocytes were stimulated ex vivo for 5 h and then gated on the CD3+CD4+ population. d The percentages of CD4+IFN-γ+ and CD4+IL-17+ cells were assessed. Values are expressed as the mean ± SEM (n = 6 each group), and Student’s t test was used to compare differences between groups. *P < 0.05; ns no significance

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