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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense

Fig. 4

The effects of the GPER agonist, G1 upon IL1RA immunoreactive protein levels in the hippocampal CA1 region following GCI. a Representative images of double-immunofluorescent staining for a IL1RA (green) and CD11b (red), b IL1RA (green) and NeuN (red). Co-localization of IL1RA immunoreactive protein with the microglia marker, CD11b, and neuronal survival marker, NeuN, is shown in magnification images. DAPI (blue) counter stain for the cell nucleus. c Western blot analysis for IL1RA. #P < 0.05 vs. 14-day IR group, ##P < 0.05 vs. G1-treated group. n = 4 in sham and G1 groups and n = 5 in IR and G36 + G1 groups. d, e The specificity of IL1RA antibody was addressed using Western blot and immunofluorescent staining of IL1RA, and DAPI (red) counter stain for cell nucleus. fh Effect of G1 on IL1RA protein expression in primary hippocampal neurons. Representative photographs of double-immunofluorescence staining for IL1RA (green) and MAP 2 (red) at 6 h after G1 or G1 + G36-administration, DAPI (blue) counter stain for cell nucleus (f). Western blot analysis showed the IL1RA protein expression in indicated groups (g, h). × 40 magnification, scale 50 μm

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