Fig. 6

Microglia density increases in the posterior hippocampus over time following mild TBI. Microglia in pig coronal sections containing posterior hippocampus (a) with an enlarged call out box (b). The ML, GCL, and Hilar region of the hippocampus were identified for each specimen and a representative image of Iba-1 staining for each experimental group is displayed (c–g) (scale = 200 μm). Microglia cell counts are quantified and graphed (h–j). Like the anterior hippocampus, biphasic trends can be observed as microglia tended to increase at 3 DPI, then decrease at 7 DPI, and finally increase again at chronic timepoints. i There is a significant increase in microglia in the GCL at 30 DPI (p = 0.0365) and 1 YPI (p = 0.0364) compared to sham