Fig. 6
HExos shifted microglia/macrophage polarization from M1 to M2 phenotype by delivering miR-216a-5p in vivo. a and c The concentrations of pro-inflammatory and anti-inflammatory cytokines in miR-NCOE-HExos, miROE-HExos, miR-NCKD-HExos and miRKD-HExos groups (n = 8/group). b and d The mRNA expression levels of M1- and M2-related genes were detected by qRT-PCR (n = 8/group). e and f The protein levels of M1- and M2-related genes were detected by western blot analysis (n = 8/group). g Representative immunostaining image of Iba1 (red) and iNOS/Arg1 (green) in the injured spinal cord lesion areas at day 3 post-injury and the analysis of iNOS/Arg1-positive microglia/macrophage in the traumatic lesion area (n = 8/group). *P < 0.05