Fig. 2

CXCR2⁺ neutrophils mediate spinal cord neuronal abnormalities. a Representative brightfield images of Golgi-Cox staining in the lumbar spinal cord (L4–L6) from control naïve mice, control mice induced with high-dose EAE, and Cxcr2 cKO mice induced with high-dose EAE. Ventral horn region of interest is identified by red dotted lines. b Representative low magnification confocal reflection images of Golgi-Cox stained spinal cord ventral horn. c Representative images of volume-rendered neuron soma. d Quantitative analysis of neuron soma volume derived from surface rendering of Golgi-Cox-stained neurons, as shown in c. A total of 628 neuron somas were included in our analyses (naïve, 133 neurons; control EAE, 254 neurons; Cxcr2 cKO EAE, 241 neurons) using the surface rendering function (Imaris). e Representative raw and rendered CRSR images of Golgi-Cox-stained neuron dendrites. f Quantitative analysis of dendritic spine density. A total of 220 dendrites were included in our analyses (naïve, 60 dendrites; control EAE, 80 dendrites; Cxcr2 cKO EAE, 80 dendrites). Control naïve, n = 3; control EAE, n = 4; Cxcr2 cKO EAE, n = 4. *p < 0.05, two-tailed unpaired Student’s t test