Fig. 2

Blimp1-deficient Tregs are unstable and convert into T_H17-like cells in EAE mice. a Frequency of splenic (SP) and spinal cord (SC) FoxP3⁺ Tregs from mice at day 20 post-EAE induction, as in Fig. 1a. b Expression of each molecule in SP and SC FoxP3⁺ Tregs from mice in a. c Expression of CD73 and FR4 in FoxP3⁺ Tregs (top) and the ratios of non-anergic (CD73⁻FR4⁻) to anergic (CD73⁺FR4⁺) population (bottom). d Production of IL-17A and GM-CSF by FoxP3⁺ Tregs. e Frequency of GM-CSF⁺ and IL-17A⁺ FoxP3⁺ Tregs from mice in a. WT, FoxP3^YFP-Cre; KO, Prdm1^fl/flFoxP3^YFP-Cre. Data represent one of at least three experiments (a, c, e n = 5/group). *P < 0.05, **P < 0.01, and ****P < 0.0001 (unpaired two-tailed Student’s t test). Bars, mean ± SEM