Fig. 7

MC1R activation on immunofluorescence staining of IL-1β and MPO at 48 h post-HI. Representative microphotographs of immunofluorescence staining and quantification of a, c IL-1β-positive cells and b, d MPO-positive cells. The number of IL-1β-positive cells or MPO-positive cells was significantly increased in the vehicle group compared to the sham group. BMS-470539 treatment significantly suppressed neuroinflammation, while these effects were reversed by knockout of MC1R or Nurr1 with CRISPR. IL-1β and MPO were green. Blue was for DAPI. Scale bar = 100 μm. Data was represented as mean ± SD. The one-way ANOVA was followed by Tukey’s post hoc test (*p < 0.05 versus sham; ^#p < 0.05 versus HI + vehicle; ^@p < 0.05 HI + BMS-470539 or HI + BMS-470539 + control CRISPR; n = 6 per group)