Fig. 8

mGluR5 exerted the anti-inflammatory effects via the modulated mGluR5-α-syn interaction in vivo. a, b Brain lysates from an LPS-induced rat PD model were immunoprecipitated with control mouse IgG or anti-α-syn, and the coprecipitated proteins were analyzed by immunoblotting with anti-mGluR5 (a). Quantification of immunoprecipited mGluR5 level was normalized to IgG and represented as the fold different ratio (lesioned/intact) of the sham group (b). c Immunofluorescent co-stainings with mGluR5 (red), α-syn (green), LAMP-1 (blue), and Iba-1 (gray) in the lesioned SN sections. Arrowheads show examples of co-localization. Scale bar, 10 μm. Rats injected with vehicle served as sham. ***p < 0.001 versus sham group; ^##p < 0.01 versus LPS lesioned group. d, e Brain lysates from an AAV-α-syn-induced rat PD model were immunoprecipitated with control mouse IgG or anti-α-syn, and the coprecipitated proteins were analyzed by immunoblotting with anti-mGluR5 (d). Quantification of immunoprecipited mGluR5 level was normalized to IgG and represented as the fold different ratio (lesioned/intact) of the AAV-GFP group (e). f Immunofluorescence of mGluR5 (red), α-syn (green), LAMP-1 (blue), and Iba-1 (gray) in the lesioned of SN showing the protein expression and co-localization from indicated treatments. Arrowheads indicate examples of co-localization. Scale bar, 10 μm. Vehicle groups with AAV-GFP virus delivery served as control. ***p < 0.001 versus AAV-GFP delivery; ^###p < 0.001 versus the AAV-α-synuclein (α-syn) delivery; ns, not significant. Data shown in all panels in this figure represent mean ± SD (n ≥ 6). The statistical significance was determined using one-way ANOVA followed by Dunnett’s test