Fig. 4

AQP4 expression (vehicle n = 5, LPS n = 5) and polarization (vehicle n = 14, LPS n = 13), as well as GFAP expression (Ctrl n = 5, LPS n = 5) after 3 h of systemic LPS injections. a AQP4 and Glut1 show cerebral vessels. b AQP4 polarization index differences were not observed 3 h after LPS. Unpaired t test, 95% CI of difference = − 0.67 to 1.8. c AQP4 detected bands at 30 kD. d Changes in AQP4, measured with Western blot, were not observed 3 h after LPS. Unpaired t test, 95% CI of difference = − 0.032 to 0.044. e GFAP showed bands at 52 kD. f GFAP was not significantly upregulated in LPS-treated mice. Unpaired t test, 95% CI of difference = − 0.26 to 0.91. Scale bars 10 μm