Fig. 5

Markers of microglia, BBB permeability and cytokines. a Iba1⁺ cells in coronal cortical sections. Scale bar = 50 μm. b Statistical analysis of Iba1⁺ cell numbers 3 h after LPS injection. Vehicle group n = 13, LPS n = 14. Unpaired t test, 95% CI of difference = − 36 to 150. c Statistical analysis of Iba⁺ area in LPS injected animals. Unpaired t test, 95% CI of difference = 0.078 to 17. d Galectin-3 detects bands at 30 kD in both groups (vehicle n = 5, LPS n = 5). e Statistical analysis of galectin-3 WB, 3 h after systemic LPS exposure. Unpaired t test, 95% CI of difference = − 1.5 to 0.56. f Claudin-5 label bands at 23 kD. Vehicle n = 5, LPS n = 5. g Statistical analysis of Claudin-5 WB. Unpaired t test, 95% CI of difference = − 0.32 to 0.12. h IgG labeling reveal cortical vessels. Scale bar = 500 μm. i Statistical analysis of IgG intensity. Vehicle group n = 5, LPS n = 5. Unpaired t test, 95% CI of difference = − 12 to 7.1. j IL-10 mesoscale finds eight samples within detection range (vehicle n = 5, LPS n = 3). Treatment differences with unpaired t test, 95% CI = − 0.31 to 0.26. Data are shown as mean and plotted individual samples, P values for group comparisons are shown above brackets