Fig. 6

Gestational sepsis downregulates synaptophysin and PSD95 expression and induces immune activation in adult offspring brains (P60). a–d Western blot was performed to detect synaptophysin and β-tubulin in the hippocampus (a), neocortex (b), frontal cortex (c), and cerebellum (d). Each bar is the mean +/− SEM from 4 to 7 animals. e–h Western blot was performed to detect PSD95 and β-tubulin in the hippocampus (e), neocortex (f), frontal cortex (g), and cerebellum (h). Each bar is the mean +/− SEM from 3 to 4 animals. i–l The immune response at P60 was evaluated through TNF-α, IL-1β, and IL-6 levels analyzed by ELISA in the hippocampus (i), neocortex (j), frontal cortex (k), and cerebellum (l). Each bar is the mean +/− SEM from 5 to 9 animals. *p < 0.05 compared saline to sepsis. NS not significant