Fig. 6

Secretion of cytokines by pro-inflammatory^LPS/IFN-γ and anti-inflammatory^IL-4/IL-13-polarized macrophages following treatment with CRYAB. a mRNA levels of iNOS, IL-12p40, arginase-1, and IL-10 in LPS/IFN-γ- and IL-4/IL-13-polarized macrophages; representative of 3 experiments, n=3–4 wells/group. b–i Concentration of IL-6 (b, f), IL-1β (c, g), IL-12p40 (d, h), and TNF-α (e, i) secreted by pro-inflammatory^LPS/IFN-γ (b–e) and anti-inflammatory^IL-4/IL-13 (f–i) -polarized macrophages from WT (white bars) and Cryab^−/− (black bars) animals grown for 48 h in the presence or absence of a CRYAB peptide; representative of 3 experiments, n=4 wells/group. Data represent mean ± sem, *p< 0.05 independent t-test