Fig. 1

L-AAA reduces the density of astrocytes but does not affect the number of neurons. Representative images and quantification of astrocytes and neurons in OBCSs treated with 1mM L-AAA or vehicle for 24 h. a–d GFAP expression in the hippocampus of OBCSs from WT and 5XFAD mice treated with 1mM L-AAA or vehicle for 24 h. e–h Expression of NeuN in the hippocampus of OBCSs from WT and 5XFAD mice. i Quantification of % area stained with GFAP (n=15–16). j Quantification of % area stained with NeuN (n=4–5). Values shown in graphs represent the mean value ± SEM. Statistical analysis included a Student’s t test, **P<0.01 ***P<0.001. Scale bar = 100 μM