Fig. 3

Effect of FIRS and postnatal inflammation on NFκB pathway gene expression and protein in the hippocampus at P7. Rat pups were exposed to prenatal i.a. LPS (L) or saline (S) (listed first) and then postnatal i.p. LPS or saline at P5 (listed second) to create four treatment groups. a Relative gene expression of indicated NFκB pathway genes in the hippocampus at P7 normalized to ribosomal protein S18 (n = 5–7/group). Protein product of each gene is in parentheses. b Western blot analyses of nuclear and cytosolic protein fractions of NFκB p65 in the hippocampus at P7 (n = 3–6/group). c Western blot analyses of nuclear and cytosolic protein fractions of NFκB p50 in the hippocampus at P7 (n = 4–6/group). d Western blot analyses of cytosolic fractions of IκBα and IκBβ proteins in the hippocampus at P7 (n = 3–5/group). In all graphs, bars above the graphs indicate significant differences between groups (p < 0.05). Data presented as mean ± SEM