Fig. 5

Effect of FIRS and postnatal inflammation on astrocytes in the hippocampus at P7. Rat pups were exposed to prenatal i.a. LPS (L) or saline (S) (listed first) and then postnatal i.p. LPS or saline at P5 (listed second) to create four treatment groups. a On P7, reactive astrocytosis was quantified in each treatment group by calculating areal coverage ratio of Gfap+ cells in the hippocampus. Scale bar represent 100 μm. Pictures representative of each group are shown at × 20. b Expression of reactive astrocyte genes in the hippocampus at P7. Bars above the graphs indicate significant differences between groups (p < 0.05). Data presented as mean ± SEM