Fig. 8

Anterograde transport of SP is reversed by INCB3344 treatment in CFA-treated animals. Ligature of the ipsilateral sciatic nerve was performed 8 days after CFA or saline administration. Forty-eight hours after ligature, the sciatic nerve was harvested for immunohistochemistry. A representative slice of SP immunoreactivity (IR) can be seen in the upper panel, showing both peripheral and central side of the constriction site. Representative photomicrographs of the central accumulation of SP-IR or CCL2-IR in sham or CFA animals, with or without INCB3344 treatment can be seen in the following smaller insets. The fluorescence intensities of the central side of SP and CCL2-IR were also quantified by measuring the fluorescence intensity in the selected region of interest (ROI). Scale bar represents 60 μm. Data correspond to mean ± SEM. We analyzed 10 nerve sections per animals with three animals per condition. *P < 0.05 compared to Sham + INCB3344. ^#P < 0.05, ^##P < 0.01 compared to CFA + saline; one-way analysis of variance followed by Bonferroni post-test