Fig. 7

4-EG induces HO-1 expression in vivo and in vitro. a The spinal cords harvested from vehicle- and 4-EG (100 mg/kg)-treated C57BL/6 EAE mice (n=5/group) at day 12–13 post-immunization were subjected to western blot analysis to measure Nrf2 and HO-1 expression. The level of Nrf2 and HO-1 expression was quantified and then normalized with β-actin, and the fold changes compared with vehicle are shown. *p<0.05 and **p<0.01 by Mann-Whitney U test. Data are representative of two independent experiments (n=5/group per experiment). b MG, BV2 cells, and macrophages were pretreated with 4-EG 400 μM for 1 hour followed by LPS 100 ng/ml stimulation for a time course. Cells were then harvested and subjected to Q-PCR analysis for HO-1, Gclc, and NQO1 mRNA expression. Data are representative of 3–4 independent experiments (n=3/technique replicates per experiment). Statistical significance was determined as *p<0.05, **p<0.01, and ***p<0.001 by one-way AVONA test