Fig. 3

Expression of inflammation resolution-associated molecules in macrophages is downregulated in a high salinity environment. a Brain sections were collected from ND and HSD mice at 3 days after 60-min tMCAO. Expression of CD16 (green) or CD206 (green) in Iba1⁺ cells (a marker of microglia and macrophage) was analyzed with immunostaining in vivo. N = 3 mice per group. **P < 0.01, ***P < 0.001, and ****P < 0.0001 versus ND group in t test. b, c Bone marrow-derived primary cultured macrophages were treated with 40 mM of NaCl overnight at the presence of LPS (100 ng/ml) or IL-4 (20 ng/ml) in vitro. Expression of CD206, Arg1, and TNFα was assessed with RT-PCR (b) and flow cytometry (c). Comparable cell counts were analyzed among the PBS, HS, PBS+LPS, HS+LPS, PBS+IL-4, and HS+IL-4 groups. Data were collected from 3 independent experiments. *P < 0.05 and **P < 0.01 versus PBS group in t test. LPS lipopolysaccharide, STR striatum, CTX cortex