Fig. 5
Excess salt downregulates efferocytic molecule TREM2 in macrophages and impairs their inflammation resolution functions. a Macrophages cultured in a high-salt environment were subjected to a PCR array to analyze the expression of efferocytosis-associated receptors in vitro. Data are displayed as fold change relative to macrophages from the PBS-treated group. Experiments were repeated three times. **P < 0.01 versus PBS group in t test. b, c Protein expression of TREM2 in macrophages after HS treatment was evaluated with western blot (b) and flow cytometry (c) in vitro. Experiments were repeated three times. *P < 0.05 versus PBS-treated group in t test. d–g ND and HSD mice were subjected to 60min of tMCAO. The brains were collected at 3 days after tMCAO. d mRNA expression of Trem1 and Trem2 in the ipsilateral hemisphere of stroke mice was analyzed with RT-PCR in vivo. CT value was normalized to that of ND mice. N = 3 mice per group. *P < 0.05 versus ND group in t test. e Protein expression of TREM2 in the ipsilateral hemisphere of stroke mice with western blot. N = 6 mice per group. *P < 0.05, versus ND group in t test. f In vivo, TREM2 protein expression in infiltrated macrophages (CD45hiCD11b+Ly6G-) of stroke mice was analyzed with flow cytometry. N = 4 mice per group. *P < 0.05 versus ND group in t test. g In vivo CD16 and CD206 expression in TREM2lo and TREM2hi macrophages (CD45hiCD11b+Ly6G-) in the ipsilateral hemisphere from ND and HSD mice was measured via flow cytometry. The mean fluorescence intensity (MFI) of CD16 or CD206 was quantified. N = 4 mice per group. *P < 0.05 and **P < 0.01 versus TREM2lo macrophages in t test