Fig. 2

Inhibition of ASIC3 attenuated mechanical allodynia and decreased TRAF6 signaling. a Amiloride improved the paw withdrawal threshold (n = 10, ^&p < 0.05, ^&&p < 0.01 vs. RTX-PHN+PBS; *p < 0.05, **p < 0.01 vs. RTX-PHN+PBS; ^#p <0.05, ^##p <0.01 vs. RTX-PHN+PBS; two-way repeated measures ANOVA followed by Bonferroni’s post hoc test). b Amiloride at 100 μg had no significant effects on the mechanism threshold in healthy control rats(n = 10). c Amiloride (100 μg) administration with once per day for 7 consecutive days effectively blocked the decreased in paw withdrawal threshold (n = 10; **p < 0.01 vs. vehicle; ^#p <0.05, ^##p < 0.01 vs. RTX-PHN; two-way repeated measures ANOVA followed by Bonferroni’s post hoc test). d-i protein expression of TRAF6, nNOS, c-Fos, BDNF, and TrkB.T1 in DRG (n = 4, *p < 0.05, **p < 0.01 vs. RTX-PHN; ^#p < 0.05, ^##p < 0.01, vs. RTX-PHN+Ami; one-way ANOVA followed by Tukey’s post hoc test)