Fig. 4

Inhibition of TrkB.T1 attenuated mechanical allodynia and decreased ASIC3 signaling. a ANA-12 increased paw withdrawal threshold (n = 10; **p < 0.01, ^∆∆p < 0.01 vs. vehicle; ^#p <0.05, ^##p < 0.01 vs. RTX-PHN; two-way repeated measures ANOVA followed by Bonferroni’s post hoc test). b ANA-12 had no significant effects on the mechanism threshold in healthy control rats (n = 10). c–g Protein expression of ASIC3, TRAF6, nNOS and c-Fos in DRGs (n = 4, *p < 0.05, **p < 0.01 vs. vehicle, ^#p <0.05, ^##p <0.01 vs. RTX-PHN; one-way ANOVA followed by Tukey’s post hoc test)