Fig. 1
TNF-α acutely enhanced ASIC-mediated currents in rat DRG neurons. a Representative current traces were evoked by application of a pH 6.0 acidic solution for 5 s in a tested DRG neuron in the presence of AMG9810 (5 μM). The low pH-induced current (IpH6.0) could be blocked by broad-spectrum ASIC channel blocker amiloride (Amil, 10 μM) and ASIC3 blocker APETx2 (2 μM). Capsaicin (Cap, 100 nM) failed to evoke any membrane currents in the presence of AMG9810 (5 μM). All membrane potentials were clamped at −60 mV. B. The sequential current traces illustrated that the amplitude of IpH6.0 progressively increased after different doses of TNF-α was pre-treated to a representative DRG cell. C. The graph showed TNF-α dose-dependently increased IpH6.0 with an EC50 of 1.96 ± 0.15 ng/ml. Each point represents the mean ± SEM of 7–10 cells