Fig. 1

Flow cytometry reveals different inflammatory profiles between sexes at 3-DPI. a Myeloid cells were isolated from the injured spinal cords of male and female, 4- and 14-MO mice at 3-DPI using a consistent gating strategy. b Representative flow plots of cells that were immuno-labeled for selection markers against all myeloid cells (CD11b and CD45) and neutrophils (Ly6G). c Female mice presented with a larger proportion of MDMs compared to both microglia (p<0.0001), and total myeloid cells (p<0.01), with a main effect of sex for total MDM events (p<0.05). c Male mice presented with trends towards a larger proportion of microglia to total myeloid cells (p=0.053). Sample size include: 4-MO female (n=3), 4-MO male (n=3), 14-MO female (n=4), 14-MO male (n=6). Main effects were assessed using two-way ANOVA, followed by pairwise comparisons using Sidak’s for post hoc analysis when appropriate. All graphs present the standard error of measurement (SEM). *p<0.05, **p<0.01