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Table 1 Gene expression differences. RNA obtained from FACS sorted MDMs and microglia were compared between cell type, sex, and age. Estimated marginal means (EMM) were obtained following MANOVA to control for the combined variable when testing sex and age, and the EMMs were used for comparisons using T test (p) with, and without, false discovery rate (FDR) correction (q) using the two-stage step-up method of Benjamini, Krieger, and Yekutieli. Genes with fewer than 75% of samples testing below detectable threshold were not compared. Genes are organized based on affiliated function as previously described [12]. Significant p and q values were set to 0.05

From: Acute inflammatory profiles differ with sex and age after spinal cord injury

Gene Common names Cell type MDMs Microglia
Sex Age Sex Age
p q p q p q p q p q
M1 activation CD80 Cluster of differentiation 80 0.005* 0.001* 0.004* 0.057 0.788 1.000 0.049* 0.225 0.678 0.996
CD86 Cluster of differentiation 86 0.001* 0.001* 0.526 0.897 0.059 0.981 0.534 0.726 0.904 1.000
FCGR1 FC gamma receptor 1 (CD64) 0.005* 0.001* 0.087 0.396 0.855 1.000 0.936 0.940 0.830 0.996
IL1b Interleukin 1β 0.001* 0.001* 0.458 0.894 0.538 1.000 Below threshold Below threshold
IL4Ra Interleukin 4 receptor a 0.001* 0.001* 0.919 0.962 0.584 1.000 0.604 0.735 0.396 0.996
TNF Tumor necrosis factor 0.361 0.044* 0.370 0.894 0.562 1.000 0.866 0.909 0.252 0.996
STAT1 Signal transducer and activator of transcription 1 0.253 0.032* 0.340 0.894 0.430 0.981 0.683 0.760 0.527 0.996
SOCS3 Suppressor of cytokine signalling 3 0.472 0.055 0.812 0.962 0.983 1.000 0.987 0.950 0.423 0.996
M2 activation STAT3 Signal transducer and activator of transcription 3 0.001* 0.001* 0.873 0.962 0.334 0.981 0.600 0.735 0.559 0.996
ARG1 Arginase-1 0.001* 0.001* 0.876 0.962 0.369 0.981 0.025* 0.195 0.955 1.000
CHI3L3 Chitinase 3-like-3 0.001* 0.001* 0.952 0.962 0.789 1.000 0.484 0.726 0.755 0.996
MANF Mes. astrocyte-derived neurotrophic factor 0.001* 0.001* 0.322 0.894 0.678 1.000 0.408 0.673 0.741 0.996
MRC1 Mannose receptor C-type 1 0.001* 0.001* 0.698 0.908 0.267 0.981 0.003* 0.036* 0.637 0.996
PPAR훾 Peroxisome proliferator activated receptor-훾 0.001* 0.001* 0.177 0.689 0.321 0.981 Below threshold Below threshold
STAT6 Signal transducer and activator of transcription 6 0.001* 0.001* 0.041* 0.277 0.391 0.981 0.375 0.666 0.023* 0.308
TGFβ1 Transforming growth factor β-1 0.001* 0.001* 0.052 0.283 0.323 0.981 0.069 0.264 0.362 0.996
ROS SOD2 Superoxide dismutase 2 0.02* 0.003* 0.523 0.897 0.959 1.000 0.144 0.333 0.466 0.996
CYBB Cytochrome b-245 (NOX2) 0.001* 0.001* 0.696 0.908 0.450 0.981 0.003* 0.036* 0.024* 0.308
EPAS1 Hypoxia-inducible factor 2-α 0.001* 0.001* 0.631 0.908 0.990 1.000 Below threshold Below threshold
HIF1a Hypoxia-inducible factor 1-α 0.001* 0.001* 0.015* 0.138 0.135 0.981 0.131 0.333 0.582 0.996
HMOX1 Heme oxygenase-1 0.001* 0.001* 0.230 0.785 0.351 0.981 0.210 0.441 0.434 0.996
IKBKB Inhibitor of NFKB 0.001* 0.001* 0.904 0.962 0.450 0.981 0.100 0.329 0.780 0.996
NFE212 Nuclear factor erythroid 2-related factor 2 (NRF2) 0.018* 0.003* 0.570 0.908 0.989 1.000 0.513 0.726 0.799 0.996
SOD1 Superoxide dismutase 1 0.001* 0.001* 0.446 0.894 0.836 1.000 0.047* 0.225 0.513 0.996
UCP2 Uncoupling protein 2 0.002* 0.001* 0.433 0.894 0.669 1.000 0.138 0.333 0.791 0.996
Debris C1qa Complement 1 subunit a 0.001* 0.001* 0.001* 0.018* 0.978 1.000 0.342 0.659 0.941 1.000
cPLA2 Phospholipase A2 0.001* 0.001* 0.685 0.908 0.187 0.981 0.691 0.760 0.724 0.996