Fig. 5

TAB2 is the functional target of miR-155. a Predicted miR-155 binding site in 3’ UTR of TAB2. Seven mutated nucleotides of the target site were indicated in red. b HEK-293T cells were co-transfected with miR-155 mimics or miR-ctrl, along with the wild-type or mutated TAB2 3’ UTR luciferase reporter plasmid, and assessed for luciferase activity at 24 h after transfection. Relative luciferase activity was expressed as the Renilla luciferase activity normalized to firefly luciferase activity. c and d The mRNA and protein levels of TAB2 were determined in U251 cells after 24 h of transfection with miR-155, anti-miR-155, or their corresponding control oligonucleotides. e and f The mRNA and protein levels of TAB2 were determined in PCN033-infected U251 cells. g U251 cells were transfected with anti-miR-155 or anti-miR-ctrl and then infected with PCN033 for 3 h. The expression of TAB2 was determined by Western blotting. h The mRNA and protein levels of TAB2 were determined in U251 cells after 24 h of transfection with siRNA-TAB2 or negative control (NC). i U251 cells were transfected with siRNA-TAB2 or NC and then infected with PCN033; the protein levels of NF-κB p65 and phosphorylated p65 were determined by Western blotting. j U251 cells were transfected with anti-miR-ctrl, anti-miR-155, or anti-miR-155, along with siRNA-TAB2, and then infected with PCN033 for 3 h. The expression of IL-1β, IL-6, TNF-α, MCP-1, and MIP-2 was determined by qPCR. All data were represented as mean + SD (n = 3/group). Statistical analysis was carried out by Student’s t test or one-way ANOVA. p < 0.05 was considered statistically significant; p < 0.01 and p < 0.001 indicated extremely significant differences