Fig. 7
The effects of Tβ4 intervention and inflammatory pathway inhibition on neuronal survival and neurotransmitter release-related proteins. a Representative photomicrographs of Nissl staining. b Histograms are the quantitative analysis of the number of survival neurons in the PtA and hippocampus. The data are presented as mean ± SEM (n = 3/group) in the different experimental groups. White dashed line implicates separated cortex layers, and white dotted circle refers to neuronal loss, with black arrow pointing to the location. c Representative photomicrographs of Map2 DAB staining and d Map2 immunoreactivity in the PtA and CA1 region. The data are presented as mean ± SEM (n = 4/group) in the different experimental groups. e The protein levels of Synapsin1, PSD95, GLUR1, 5-HT1AR, and CREB in the cortex and hippocampus were measured by western blotting. f–j The measurement of gray intensity of these proteins in the cortex and hippocampus. The data are presented as mean ± SEM (n = 5/group) in the different experimental groups. #p < 0.05, ##p < 0.01 vs WT mice; *p < 0.05, **p < 0.01 vs APP/PS1 mice