Fig. 1

Detection of LACV in BCEC preps from weanling but not adult mice ex vivo. (a) Whole brain BCECs were isolated at 1, 2, and 3 dpi from weanling and adult LACV or mock-infected mice (N = 3 to 6 for each dpi) and then analyzed for LACV RNA. A Ttwo-way ANOVA was performed for understanding the difference in time and age. *P < 0.05, ****P < 0.0001. (b–j) Whole brain BCECs, isolated from weanling and adult LACV and mock-infected animals, were cultured until confluence. (b) Supernatants from LACV-infected weanling and adult BCEC were analyzed for infections virus. (*P < 0.01 between adult and weanling LACV, one-way ANOVA, I). Data are the mean from 3 samples. (c–j) Cells were immunostained with LACV (c–e) and ZO1 (f–h) or claudin 5 (cldn5, i and j). Scale bar = 100 μm (c–e) and scale bar =20 μm (f–j). Each symbol represents data obtained from a single mouse BCEC