Fig. 4

Effect of Ac2-26 on BBB disruption and neuronal apoptosis in the peri-infarct cortex at 3 days post-tMCAO/R. a Representative photographs of immunostaining and quantitative analyses of EB dye extravasation. White arrows indicated exudative EB dye. Scale bar = 100 μm. b Representative western blotting bands and densitometric quantifications of albumin extravasation and TJ (occludin and claudin-5) expressions. c–d Representative photographs of immunostaining and quantitative analyses of TJ (occludin and claudin-5; red) expression and neuronal apoptosis (NeuN, red; TUNEL, green). Scale bar = 200 μm. Data were presented as the mean ± SD (n = 6/group), and were analyzed by independent samples t test. *p < 0.05, **p < 0.01, and ***p < 0.001