Fig. 5

Effect of FPR2/ALX-dependent AMPK-mTOR pathway on Ac2-26-mediated microglial/macrophage polarization in the peri-infarct cortex at 3 days post-tMCAO/R. a Neurological function was evaluated by mNSS test. b Representative nissl staining and quantitative analyses of cerebral infarct volume. c Quantitative analyses of EB dye extravasation. d Representative western blotting bands and densitometric quantifications of activated microglial/macrophage marker Iba-1, M1-phenotype marker CD16, and M2-phenotype marker CD206. e qRT-PCR analyses of mRNA expressions of M1-phenotype markers (CD16, CD32, and iNOS) and M2-phenotype markers (CD206, Arg-1, and YM1). f ELISA analyses of the expressions of a pro-inflammatory cytokine IL-1β (M1-phenotype) and an anti-inflammatory cytokine IL-10 (M2-phenotype). g Representative western blotting bands and densitometric quantifications of phosphorylation of AMPKα and mTOR. Data were presented as the mean ± SD (A n = 12/group; b–g n = 6/group) and were analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison test. *p < 0.05, **p < 0.01, and ***p < 0.001, ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001