Fig. 4
Nlrp3 knockout failed to reduce mice death which was caused by mere heat treatment. The experiment was designed into four groups: control (WT), control (Nlrp3-/-), no LPS/heat (WT), and no LPS/heat (Nlrp3-/-). The comparison was conducted between Nlrp3-/- and wild-type mice. Hypothalamus IL-1β, IL-6, and TNF-α was measured by ELISA. a Survival curve was monitored. The statistical analysis was conducted by Log-rank test (n = 20). b Core temperature (TC) profiles of mice were monitored in every 15 min. The average time taken by core temperature to reach 42 °C was calculated by mean ± SEM. (n = 5 to 10). c Tissue homogenate level of IL-1β was measured. d Tissue homogenate level of IL-6 was measured. e Tissue homogenate level of TNF-α was measured. Each cytokine concentration were expressed as the mean ± SEM. (n = 5 to 10). f Immunohistochemical result of IL-1β between control (WT) vs no LPS/Heat (WT) group mice. Three representative pictures were selected. g Quantification and statistical analysis of the immunohistochemical microscopies. (n = 5–10) **p < 0.01. nsp > 0.05 (ns: no significance)