Fig. 5
Nlrp3 knockout protected mice from heat stroke-induced mice death by inhibiting IL-1β releasing. The experiment was designed into four groups: control (WT), control (Nlrp3-/-), LPS/heat (WT), and LPS/heat (Nlrp3-/-).LPS treatment condition was 1 mg/kg and whole-body heating (WBH) temperature was 41.2 °C. Systemic pro-inflammatory cytokines and hypothalamusIL-1β were measured by ELISA and immunohistochemistry, respectively. The comparison was conducted between Nlrp3 knock out and wild-type mice. a Survival curve was monitored. The statistical analysis was conducted by Log-rank test (n = 20). b Core temperature (TC) profiles of mice were monitored in every 15 min. The average time taken by core temperature to reach 42 °C was calculated by mean ± SEM. (n = 5 to 10). c Serum level of pro-inflammatory cytokines IL-1β was measured by ELISA. d Serum level of pro-inflammatory cytokines IL-6 was measured by ELISA. e Serum level of pro-inflammatory cytokines TNF-α was measured by ELISA. f Hypothalamus IL-1β were measured immunohistochemistry, the H-score method was conducted for the staining quantitation. Each cytokine concentration and immunohistochemistry quantitation were expressed as the mean ± SEM. (n = 5 to 10). **p < 0.01, *p < 0.05