Fig. 6
IFN-I signal-incompetent mice show impaired innate IFN-I responses. a and b The expression of IFN-I (IFN-α/β) mRNA in the peripheral and CNS tissues. c and d Impaired induction of RIG-I-like receptors (RLR) and IRF genes in the peripheral and CNS tissues of IFN-I signal-incompetent mice. e Impaired expression of IFN-stimulated genes (ISGs) in the peripheral and CNS tissues of IFN-I signal-incompetent mice. The mRNA levels of the indicated genes were determined by real-time qRT-PCR using total RNA extracted from popliteal LNs (pLN), spleen, mesenteric LNs (mLN), iliac LNs (iLN), liver, spinal cord (SC), and brain at the indicated days after JEV infection (5 × 106 ffu/mouse) via the footpad route. Data in the graphs denote the mean ± SEM. Results are representative of one out of at least two individual experiments with four to five mice per group. Statistical significance *p < 0.05; **p < 0.01; ***p < 0.001 was assessed by an unpaired two-tailed Student’s t test