Type I IFN signaling limits hemorrhage-like disease after infection with Japanese encephalitis virus through modulating a prerequisite infection of CD11b+Ly-6C+ monocytes

Patil, Ajit Mahadev; Choi, Jin Young; Park, Seong Ok; Uyangaa, Erdenebelig; Kim, Bumseok; Kim, Koanhoi; Eo, Seong Kug

doi:10.1186/s12974-021-02180-5

Journal of Neuroinflammation

Table 1 Specific primers for the expression of cytokines, chemokines, transcription factor, and JEV RNA used in real-time qRT-PCR

From: Type I IFN signaling limits hemorrhage-like disease after infection with Japanese encephalitis virus through modulating a prerequisite infection of CD11b⁺Ly-6C⁺ monocytes

Gene name^a	Primer sequence (5’-3’)^b	Position cDNA	Gene bank ID
TNF-α	FP: CGT CGT AGC AAA CCA CCA AG RP: TTG AAG AGA ACC TGG GAG TAG ACA	449-468 575-598	NM_013693.3
IL-6	FP: TGG GAA ATC GTG GAA ATG AG RP: CTC TGA AGG ACT CTG GCT TTG	256-275 489-509	NM_031168.2
IFN-α	FP: TGT CTG ATG CAG CAG GTG G RP: AAG ACA GGG CTC TCC AGA C	367-385 514-532	NM_008334.3
IFN-β	FP: TCC AAG AAA GGA CGA ACA TTC G RP: TGA GGA CAT CTC CCA CGT CAA	106-127 399-419	NM_010510.1
CCL2	FP: AAA AAC CTG GAT CGG AAC CAA RP: CGG GTC AAC TTC ACA TTC AAA G	347-367 426-447	NM_011333
CXCL2	FP: ATC CAG AGC TTG AGT GTG ACG C RP: AAG GCA AAC TTT TTG ACC GCC	194-215 263-283	NM_009140.2
RIG-I	FP: CCA CCT ACA TCC TCA GCT ACA TGA RP: TGG GCC CTT GTT GTT CTT CT	194-217 260-279	NM_172689.3
MDA5	FP: GGC ACC ATG GGA AGT GAT T RP: ATT TGG TAA GGC CTG AGC TG	1178-1196 1247-1266	NM_027835.3
IRF3	FP: GAT GGA GAG GTC CAC AAG GA RP: GAG TGT AGC GTG GGG AGT GT	1170-1189 1259-1278	NM_016849.4
IRF7	FP: CCT CTT GCT TCA GGT TCT GC RP: GCT GCA TAG GGT TCC TCG TA	980-999 1080-1099	NM_016850.3
ISG49	FP: GCC GTT ACA GGG AAA TAC TGG RP: CCT CAA CAT CGG GGC TCT	919-939 1126-1143	NM_010501.2
ISG54	FP: GGG AAA GCA GAG GAA ATC AA RP: TGA AAG TTG CCA TAC AGA AG	1434-1453 1587-1606	NM_008332.3
ISG56	FP: CAG AAG CAC ACA TTG AAG AA RP: TGT AAG TAG CCA GAG GAA GG	774-793 911-930	NM_008331.3
Claudin-1	FP: TCT ACG AGG GAC TGT GGA TG RP: TCA GAT TCA GCA AGG AGT CG	350-369 414-433	NM 016674.4
Claudin-5	FP: GTG GAA CGC TCA GAT TTC AT RP: TGG ACA TTA AGG CAG CAT CT	1054-1073 1131-1150	NM 013805.4
Occludin	FP: GCT GTG ATG TGT GTG AGC TG RP: GAC GGT CTA CCT GGA GGA AC	2054-2074 2105-2124	NM 008756.2
ZO-1	FP: AGG ACA CCA AAG CAT GTG AG RP: GGC ATT CCT GCT GGT TAC A	6227-6246 6295-6313	NM 009386.2
ICAM-1	FP: GTC CGC TGT GCT TTG AGA ACT RP: CGG AAA CGA ATA CAC GGT GAT	358-378 411-431	NM 010493.3
JAM	FP: ACC CTC CCT CCT TTC CTT AC RP: CTA GGA CTC TTG CCC AAT CC	1163-1182 1238-1257	NM 172647.2
β-actin	FP: TGG AAT CCT GTG GGA TCC ATG AAA C RP: TAA AAC GCA GCT CAG TAA CAG TCC G	915-939 1239-1263	NM_007393.5

^a IL interleukin
^b FP forward primer, RP reverse primer

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ISSN: 1742-2094

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