Fig. 7

Macrophages migrate from visceral fat pad to the hypothalamus. A Visceral fat pads that contained both donor and endogenous tissue show increase in macrophage percentage following HFD compared to control diet, regardless of donor diet. B Subcutaneous fat depot only contains recipient endogenous CD45.1, indicating that macrophages do not infiltrate subcutaneous fat depot. C Hypothalamus demonstrates increase in macrophage proportion following HFD (left panel), presence of endogenous, recipient CD45.1 (middle panel) and donor CD45.2 macrophages (right panel). D Proportion of pro-inflammatory CD11c+ macrophages and resident CD206+ macrophages within CD45.1 and CD45.2 hypothalamic macrophages. Statistical significance (*) between control-fed recipient (second letter C) and HFD-fed recipient (second letter H) were determined by ANOVA followed by Tukey’s post hoc test