Fig. 1

Examination of D-DT and MIF protein levels and colocalization with astrocytes following rat SCI. a Western blot analysis of D-DT and MIF following spinal cord injury at 0, 1, 4, and 7 days. The sham and 0-day group were used as control. b Quantification data as shown in (a). Quantities were normalized to endogenous β-actin. c Illustration of horizontal section sites at the contused cord. d–s Immunostaining showed colocalization of D-DT with GFAP- and S100β-positive astrocytes. Rectangle indicates region magnified. Arrowheads indicate the positive signals. Scale bars, 500 μm in (d), (f), (h), (j), (l), (n), (p) and (r); 50 μm in (e), (g), (i), (k), (m), (o), (q), and (s). Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)