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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Fundamentally different roles of neuronal TNF receptors in CNS pathology: TNFR1 and IKKβ promote microglial responses and tissue injury in demyelination while TNFR2 protects against excitotoxicity in mice

Fig. 3

Neuronal TNFR1 increases the induction of autophagy in corpus callosum non-neuronal cells. (A) Immunofluorescence staining of LC3B (red), and DAPI (blue) in brain coronal paraffin sections through the corpus callosum from representative nTNFR1KO and TNFR1ff control naïve (CPZ0) and CPZ-fed CPZ5 mice (n = 3 for all CPZ0 and CPZ5 time points). Scale bars: 20 μm; 60x oil immersion objective. (B) Higher power insets from CPZ-fed CPZ5 mice of each genotype shown in (A), arrowheads showing LC3B-positive puncta. (C) Quantitative analysis of LC3B-positive puncta in LC3B-immunoreactive cells (n = 50 LC3B-immunoreactive cells were measured from each mouse strain at each time point). Means ± SD LC3B puncta/cell are depicted. p-value has been calculated by one-way ANOVA

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Journal of Neuroinflammation

ISSN: 1742-2094