Fig. 5

ABCA1 is a phagocytosis-associated molecule mediated by AXL in astrocytes. (A) Expression of ABCA1 is detected after AXL intervention at 3 dpi. **p<0.01 versus sham, ^##p<0.01 versus vehicle by one-way ANOVA. (B) Immunofluorescence staining of ABCA1 (red) and GFAP (green) colocalization at 3 dpi in the ipsilateral cortex after rmGas6 treatment. (C) Immunoblot of ABCA1 in primary astrocytes with or without drug administration. *p < 0.05, **p < 0.01 by one-way ANOVA. (D) Representative Western blot images and quantitative analyses of p-STAT1/STAT1 in primary cultured astrocytes stimulated by rmGas6 at different time points. **p < 0.01 versus 0 min by one-way ANOVA. (E) Representative Western blot images and quantitative analyses of ABCA1 in primary astrocytes. **p < 0.01 versus vehicle, ^##p < 0.01 versus rmGas6 by one-way ANOVA. (F) Representative histograms show the ROS level in astrocyte samples from each group. (G) Quantification of percentage of ROS expression in astrocytes. *p < 0.05, **p < 0.01 versus sham, ^#p < 0.05, ^##p < 0.01 versus vehicle, @p<0.05 versus rmGas6 by one-way ANOVA. (H, I) Relative TNFα and IL-1β mRNA expression in astrocytes from each group. **p < 0.01 versus sham, ^#p < 0.05, ^##p < 0.01 versus vehicle, ^@p < 0.05, ^@@p < 0.01 versus rmGas6 by one-way ANOVA