Fig. 4

OZ treatment reduces neuronal pyroptosis after SAH. a Double immunostaining of NeuN and GSDMD and b quantitative analysis of GSDMD-positive neurons in ipsilateral cortex 24 h after SAH. Scale bar: 20 μm. c Immunoblot analysis of GSDMD expression in the treated mice. d Representative transmission electron micrographs of neurons in brain tissues. Red arrowhead: membrane pores. Scale bar: 2 μm. e The levels of IL-β and IL-18 in the ipsilateral cortex were analyzed by ELISA kits. f Western blot and quantitative analysis of GSDMD in the Control, OxyHb, and OxyHb+OZ groups. g The concentration of IL-1β and IL-18 in the supernatant of the cell culture. Data are expressed as mean ± SD, n = 5 per group. ***P < 0.001 vs Sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs SAH+Vehicle group. ^†††P < 0.001 vs Control group; ^§P < 0.05, ^§§P < 0.01, ^§§§P < 0.001 vs OxyHb group