Fig. 8

OZ treatment mitigates oxidative stress following SAH. a DHE staining in the ipsilateral cortex of mice brain and b quantitative analysis of DHE fluorescence intensity. c Assessment of SOD activity in brain tissues. d Treated neurons were incubated with 5 μM DCFH-DA to detect intracellular ROS levels and e quantitative analyses of the number of DCFH-DA-positive neurons. OZ administration notably reduced OxyHb- and OxyHb+BSO- induced increment of DCFH-DA-positive neurons. f Determination of SOD activity in neurons. g Western blot and quantitative analysis of NLRP3, ASC, cleaved caspase-1, IL-β in treated neurons. Data are expressed as mean ± SD, n = 5 per group. ***P < 0.001 vs Sham group; ^#P < 0.05, ^##P < 0.01 vs SAH+Vehicle group. ^†††P < 0.001 vs Control group; ^§P < 0.05, ^§§§P < 0.001 vs OxyHb group; ^$P < 0.05, ^$$$P < 0.001 vs OxyHb+BSO group. Scale bar: 50 μm