Fig. 6

CatH deficiency enhanced the proliferation of astrocytes in the hippocampus after HI. A, B Immunofluorescent CLSM images of GFAP (green) and Hoechst (blue) in the hippocampal CA1, CA3, and dentate gyrus (DG) regions of WT (A) and CatH^−/− (B) mice at 72 h after HI injury. Scale bar, 100 μm. C CLSM images of the astrocyte monolayers immune-stained by anti-GFAP antibody (green) with nuclear staining by Hoechst (blue) in the astrocyte monolayers 72 h after the scratch in the absence and presence of IFN-β. Dashed lines highlight the area that was damaged by the scratch. Scale bar, 50 μm. D Quantification of the migration of cultured astrocytes shown in (C). The gap width from three separate experiments was quantitatively evaluated using ImageJ software. The asterisks indicate a statistically significant difference from control group value (**p < 0.05, unpaired t test)