Fig. 5

Motif analysis reveals shared DNA binding sequences. A Dot plot of the significantly overrepresented motifs in DARs after CCI and CFA. The size of the circle represents the % of DARs that contain the motif and color indicates the q value. B Venn diagram that shows the overlap of the number of DNA binding motifs in the DARs between CCI and Naïve, and the CFA versus Naïve comparisons. Two motifs are common which are identified as Foxo1 and Hlf. The motif and statistics from HOMER for each of these motifs is provided. C Chromatin accessibility at a putative regulatory region of the Grik4 gene. The average ATAC-seq signal of the downsampled, normalized bigwig files for each group as displayed from the Integrated Genomics Viewer (left). The region identified as being differentially accessible in both the CCI and CFA groups compared to the Naïve group is outlined (chr8:47,237,180–47,237,538). This 358bp sequence is provided with the putative Foxo1 binding site highlighted in red. A box plot of the normalized log₂transformed ATAC-seq signal for each sample (naïve, n = 3; CFA, n = 6; CCI, n = 5). CCI chronic constriction injury; CFA complete Freund’s adjuvant; DAR differentially accessible region