Fig. 5

RSL3 inhibits cytokine gene transcription. a The qRT-PCR analysis of IL6 and IL-1b mRNA levels in RSL3 or vehicle-treated BV2 microglia subjected to ActD (1 μg/ml) treatment for the indicated times after LPS pre-treated for 2 h. b Representative immunoblot analysis of the phosphorylated (p-) or total proteins in lysates of BV2 microglia subjected to RSL3 or DMSO in the presence of LPS (100 ng/ML) for indicated times. ChIP-qRT-PCR analysis of P65 (c) and RNA Pol II (d) binding in IL-6 and IL-1b loci in BV2 microglia lysates. BV2 microglia were stimulated with LPS (100 ng/ML) or LPS plus RSL3 (500 nM) for 4 h and CHIP assay was then performed. pro, promoter; TSS, transcription start site. The data are means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 by one-way-ANOVA analysis followed by Turkey test. n.s., no significant. All data are representative of or combined from at least three independent experiments