Fig. 7

RSL3 in combination with Fer-1 mitigates LPS-induced inflammation. a Survival of C57BL/6 mice (injected with RSL3 plus Fer-1 or vehicle; n = 9 for each treatment) after lethal challenge with LPS (10 mg/kg body weight). b ELISA of TNFα, IL-6, and IL-1β in serum from C57BL/6 mice (injected with RSL3 plus Fer-1 or vehicle; n = 5 per group) after challenging with PBS or LPS for 6 h. c Hematoxylin and eosin staining of lungs from mice (injected with RSL3 plus Fer-1 or vehicle; n = 5 per group) for different treatment after challenging with PBS or LPS. Scale bar, 50 μm. d The number of microglia (CD45^intCD11b^high) in brain tissues after indicated treatment (LPS, 5 mg/kg body weight) based on flow cytometry analysis. e The qRT-PCR analysis of TNF, IL-6, and IL-1b in brain tissues under different treatment (LPS, 5mg/kg body weight). The data are means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 by one-way-ANOVA analysis followed by Turkey test. n.s., no significant. All data are representative of or combined from at least three independent experiments